国产亚洲高清在线精品不卡-国产亚洲精aa在线观看不卡-国产亚洲精彩视频-国产亚洲精品91-日本aⅴ日本高清视频影片www-日本aⅴ视频

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>AN3 CA人子宮內(nèi)膜腺癌細(xì)胞

人子宮內(nèi)膜腺癌細(xì)胞

簡要描述:HTB-111 AN3 CA 人子宮內(nèi)膜腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:AN3 CA
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-12
  • 訪  問  量:1840

產(chǎn)品分類

Product Category

詳細(xì)介紹

HTB-111 AN3 CA 人子宮內(nèi)膜腺癌細(xì)胞

ATCC® Number: HTB-111™ Price: $399.00

Designations: AN3 CA

Depositors: CJ Dawe

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Proper sapiens (human)

Morphology: epties: adherent

Organism: Homoithelial

HTB-111 AN3 CA 人子宮內(nèi)膜腺癌細(xì)胞

Source: Organ: uterus

Tissue: endometrium

Disease: adenocarcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 12,14,15

D13S317: 12,14

D16S539: 10,14

D5S818: 11,14

D7S820: 7,10,7.1

THO1: 10,9.3

TPOX: 8,10

vWA: 14,20

Isoenzymes: AK-1, 1-2

ES-D, 1

G6PD, B

GLO-I, 2

PGM1, 1

PGM3, 1-2

Age: 55 years

Gender: female

Ethnicity: Caucasian

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Volumes used in this protocol are for 75 sq. cm flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22517: Dawe CJ, et al. Growth in continuous culture, and in hamsters, of cells from a neoplasma assoicated with Acanthosis nigricans. J. Natl. Cancer Inst. 33: 441-456, 1964. PubMed: 14207855

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

29988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105



HTB-111 AN3 CA





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 亚洲毛片儿| 台湾佬自偷自拍情侣在线| 亚洲伊人tv综合网色| 奇米影视8888| 亚洲热色| 四虎91视频| 亚洲a级| 欧美一级看片免费观看视频在线| 欧美福利在线播放| 亚洲国产精品免费视频| 亚洲视频 中文字幕| 人人干人人搞| 欧美色成人综合| 天天天天天天天操| 亚洲国产精品一区二区久 | 日韩欧美亚洲精品| 色青青草原桃花久久综合| 欧美亚洲另类一区中文字幕| 亚洲精品影视| 青青青久久久| 色吧综合| 小黄色片| 四虎永久免费观看| 热99视频| 日本一区深夜影院深a| 台湾老艳片一级| 日韩精品中文乱码在线观看| 一级片网| 日韩欧美中文字幕一区| 奇米影视8888| 青青草99| 亚洲啪啪网址| 亚洲成a人不卡在线观看| 奇米久久久| 亚洲成a人| 日本玖玖| 日本不卡免费新一区二区三区| 四川一级毛片| 无遮挡一级毛片视频| 午夜激爽毛片在线看| 亚久久伊人精品青青草原2020|